Identify unsatisfied polars in H-bonds

Started by BootsMcGraw

bkoep Staff Lv 1

Yes, you are absolutely right! This is a problem with the score function of the HBond Network Objective that needs to be fixed.

BUNS (buried unsatisfied polar atoms) are only problematic when they are buried. If a H-bond network extends to the protein surface, then the exposed atoms can be satisfied by making H-bonds with water. These exposed polar atoms should not jeopardize protein folding, and they should not incur a penalty.

ichwilldiesennamen Lv 1

Thanks bkoep for outlining this point so well. This is exactly what I wanted to express. And many thanks for taking care that this gets implemented. I am really very much looking forward to this. But I guess it will be quite tricky to implement. I wish good luck to the whole team!

BootsMcGraw Lv 1

I uploaded to the scientists solution "82314 - 2102…" that shows a nitrogen from a network-bound histidine clearly sticking out into solution.

Penalized over 250 points for that. :-(

Looking forward to the Objective fix.

BootsMcGraw Lv 1

Solution "82314-2130" uploaded to scientists. The sidechain in question is GLU 18. One of the oxygens is clearly exposed to solution, yet is counted as BUNS.