Placeholder image of a protein
Icon representing a puzzle

2723: Electron Density Reconstruction 156

Closed since about 2 months ago

Novice Overall Prediction Electron Density

Summary

Created
January 27, 2026
Expires
Max points
100
Description

The rather funky structure of this protein complex has already been solved and published, but close inspection suggests that there are some problems with the published solution. We'd like to see if Foldit players can use the same electron density data to reconstruct a better model. This puzzle comes from PDB entry 2OCY.

Sequence
DSIMKILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDFKIKTVDINGKKVKLQLWDTAGQERFRTITTAYYRGAMGIILVYDVTDERTFTNIKQWFKTVNEHANDEAQLLLVGNKSDMETRVVTADQGEALAKELGIPFIESSAKNDDNVNEIFFTLAKLIQEKIDSN SMALSTQLIESVDKQSHLEEQLNKSLKTIASQKAAIENYNQLKEDYNTLKRELSDRDDEVKRLREDIAKENELRTKAEEEADKLNKEVEDLTASLFDEANNMVADARKEKYAIEILNKRLTEQLREKDTLLDTLTLQLKNLKKVMHSLDNESTV

Top groups

  1. Avatar for BOINC@Poland 11. BOINC@Poland 1 pt. 49,777
  1. Avatar for nicobul 21. nicobul Lv 1 21 pts. 53,652
  2. Avatar for g_b 22. g_b Lv 1 19 pts. 53,639
  3. Avatar for westchuck 23. westchuck Lv 1 17 pts. 53,634
  4. Avatar for dpmattingly 24. dpmattingly Lv 1 16 pts. 53,530
  5. Avatar for BarrySampson 25. BarrySampson Lv 1 14 pts. 53,506
  6. Avatar for Wanderer09 26. Wanderer09 Lv 1 13 pts. 53,443
  7. Avatar for Anfinsen_slept_here 27. Anfinsen_slept_here Lv 1 12 pts. 53,387
  8. Avatar for silent gene 28. silent gene Lv 1 10 pts. 53,367
  9. Avatar for Xendrais 29. Xendrais Lv 1 9 pts. 53,252
  10. Avatar for NinjaGreg 30. NinjaGreg Lv 1 8 pts. 52,962

Comments

LociOiling Lv 1

This puzzle seems to have a pucker due to missing residues. Segment 344 is chain C, residue 63, then segment 345 is chain C, residue 70. Segments 344 and 345 are connected, and start with large negative ideality, around -17,000 points each.

LociOiling Lv 1

There is also a problem at segments 426-428. The PDB number jumps from chain C, residue 163 at segment 426, to chain C, residue 170 at segment 427, but 426 and 427 are connected.

Then 427 is not connected to 428, but the PDB numbering says it should be. Kind of a reverse pucker there.

horowsah Staff Lv 1

Those issues now in theory should be fixed, but will have to reset the puzzle from the undo menu.

Anfinsen_slept_here Lv 1

At the discontinuity between 427-428, it looks like there is density to support adding in some of the residues missing from the published sacchromyces SEC4 sequence. I wonder if there is a way for FOLDIT central to allow us to do that?

LociOiling Lv 1

Thx Loci for the infos. So I really have to start anew after deleting that puzzle-directory? That doesn't sound like much fun.

ichwilldiesennamen Lv 1

This puzzle seems to have a pucker due to missing residues. Segment 344 is chain C, residue 63, then segment 345 is chain C, residue 70. Segments 344 and 345 are connected, and start with large negative ideality, around -17,000 points each.

ichwilldiesennamen Lv 1

Even better: such a "closed" solution cannot be uploaded or opened again anywhere else. So the only thing that seems to work is to leave the cut in. What a mess :(

LociOiling Lv 1

This puzzle seems to have a pucker due to missing residues. Segment 344 is chain C, residue 63, then segment 345 is chain C, residue 70. Segments 344 and 345 are connected, and start with large negative ideality, around -17,000 points each.

ichwilldiesennamen Lv 1

Those issues now in theory should be fixed, but will have to reset the puzzle from the undo menu.