Placeholder image of a protein
Icon representing a puzzle

360: Quest to the Native CASP9 Puzzle 3

Closed since over 15 years ago

Intermediate

Summary

Created
September 13, 2010
Expires
Max points
100
Description

We are giving you CASP9 Puzzle 3 again, but this time with the native structure as a guide. Use the alignment tool and try to match the native as much as possible. After threading, make sure to press G to Align the Guide. See the puzzle comments for additional information about this particular protein.

Top groups

  1. Avatar for Deleted group 11. Deleted group pts. 11,245
  2. Avatar for Deleted group 12. Deleted group pts. 11,153
  3. Avatar for L'Alliance Francophone 13. L'Alliance Francophone 3 pts. 11,085
  4. Avatar for Boinc.be 14. Boinc.be 2 pts. 10,978
  5. Avatar for Mojo Risin' 15. Mojo Risin' 1 pt. 10,789
  6. Avatar for Deleted group 16. Deleted group pts. 10,219
  7. Avatar for Crunching Family 17. Crunching Family 1 pt. 9,340
  8. Avatar for DSN @ Home 18. DSN @ Home 1 pt. 8,353
  9. Avatar for Mmm Helix Fries. 19. Mmm Helix Fries. 1 pt. 8,187
  10. Avatar for UCD2010 20. UCD2010 1 pt. 7,815
  1. Avatar for Datstandin 41. Datstandin Lv 1 48 pts. 11,001
  2. Avatar for Lervad 42. Lervad Lv 1 47 pts. 10,998
  3. Avatar for BootsMcGraw 43. BootsMcGraw Lv 1 46 pts. 10,997
  4. Avatar for MooMooMan 44. MooMooMan Lv 1 45 pts. 10,989
  5. Avatar for Perzik 45. Perzik Lv 1 44 pts. 10,978
  6. Avatar for itskimo 46. itskimo Lv 1 43 pts. 10,976
  7. Avatar for Dj-P 47. Dj-P Lv 1 42 pts. 10,966
  8. Avatar for anthion 48. anthion Lv 1 41 pts. 10,938
  9. Avatar for Deleted player 49. Deleted player 40 pts. 10,928
  10. Avatar for SKSbell 50. SKSbell Lv 1 40 pts. 10,921

Comments

beta_helix Staff Lv 1

The solved native structure for CASP9 Puzzle 3, target T0527, is missing 3 residues in the middle of the protein.

You can see it here:
http://www.rcsb.org/pdb/explore/images.do?structureId=3MR0
in the image on the top left, the green region has a chain break (next to the cyan/blue area).

Because of this, we have used Rosetta to fill in the 3 missing residues so that the guide has a continuous chain (since there aren't that many different places where those 3 residues could go)
These 3 residues are Glycine 47, Histidine 48, & Arginine 49.

Also, as Madde pointed out in the forum:
http://fold.it/portal/node/988108#comment-8844

the C-terminal helix points straight out, even though it has a bunch of hydrophobic residues.