Placeholder image of a protein
Icon representing a puzzle

360: Quest to the Native CASP9 Puzzle 3

Closed since over 15 years ago

Intermediate

Summary

Created
September 13, 2010
Expires
Max points
100
Description

We are giving you CASP9 Puzzle 3 again, but this time with the native structure as a guide. Use the alignment tool and try to match the native as much as possible. After threading, make sure to press G to Align the Guide. See the puzzle comments for additional information about this particular protein.

Top groups

  1. Avatar for turkish voluntered 21. turkish voluntered 1 pt. 7,793
  2. Avatar for Super Happy Fun Time 22. Super Happy Fun Time 1 pt. 2,872
  3. Avatar for Bio-Hazard 23. Bio-Hazard 1 pt. 0
  4. Avatar for Window Group 24. Window Group 1 pt. 0
  1. Avatar for Deleted player 51. Deleted player pts. 10,907
  2. Avatar for Tamison 52. Tamison Lv 1 38 pts. 10,891
  3. Avatar for ice--9 53. ice--9 Lv 1 37 pts. 10,883
  4. Avatar for fishercat 54. fishercat Lv 1 36 pts. 10,871
  5. Avatar for kleinesregenbogengesicht 55. kleinesregenbogengesicht Lv 1 36 pts. 10,864
  6. Avatar for TheGUmmer 56. TheGUmmer Lv 1 35 pts. 10,863
  7. Avatar for Museka 57. Museka Lv 1 34 pts. 10,862
  8. Avatar for aendgraend 58. aendgraend Lv 1 33 pts. 10,833
  9. Avatar for Tlaloc 59. Tlaloc Lv 1 33 pts. 10,789
  10. Avatar for G d S 60. G d S Lv 1 32 pts. 10,773

Comments

beta_helix Staff Lv 1

The solved native structure for CASP9 Puzzle 3, target T0527, is missing 3 residues in the middle of the protein.

You can see it here:
http://www.rcsb.org/pdb/explore/images.do?structureId=3MR0
in the image on the top left, the green region has a chain break (next to the cyan/blue area).

Because of this, we have used Rosetta to fill in the 3 missing residues so that the guide has a continuous chain (since there aren't that many different places where those 3 residues could go)
These 3 residues are Glycine 47, Histidine 48, & Arginine 49.

Also, as Madde pointed out in the forum:
http://fold.it/portal/node/988108#comment-8844

the C-terminal helix points straight out, even though it has a bunch of hydrophobic residues.