Placeholder image of a protein
Icon representing a puzzle

360: Quest to the Native CASP9 Puzzle 3

Closed since over 15 years ago

Intermediate

Summary

Created
September 13, 2010
Expires
Max points
100
Description

We are giving you CASP9 Puzzle 3 again, but this time with the native structure as a guide. Use the alignment tool and try to match the native as much as possible. After threading, make sure to press G to Align the Guide. See the puzzle comments for additional information about this particular protein.

Top groups

  1. Avatar for Anthropic Dreams
    1. Anthropic Dreams
    100 pts. 11,575
  2. Avatar for Contenders 2. Contenders 81 pts. 11,561
  3. Avatar for Void Crushers 3. Void Crushers 64 pts. 11,527
  4. Avatar for SETI.Germany 4. SETI.Germany 50 pts. 11,463
  5. Avatar for Natural Abilities 5. Natural Abilities 39 pts. 11,456
  6. Avatar for Go Science 6. Go Science 30 pts. 11,416
  7. Avatar for Richard Dawkins Foundation 7. Richard Dawkins Foundation 23 pts. 11,401
  8. Avatar for Russian team 8. Russian team 17 pts. 11,364
  9. Avatar for Deleted group 9. Deleted group pts. 11,302
  10. Avatar for BOINC@Poland 10. BOINC@Poland 9 pts. 11,274
  1. Avatar for prot-bustr
    1. prot-bustr Lv 1
    100 pts. 11,575
  2. Avatar for Sigian 2. Sigian Lv 1 85 pts. 11,575
  3. Avatar for itskimo 3. itskimo Lv 1 72 pts. 11,575
  4. Avatar for Ryanfav 4. Ryanfav Lv 1 61 pts. 11,573
  5. Avatar for KingFrog 5. KingFrog Lv 1 51 pts. 11,573
  6. Avatar for CaNNon 6. CaNNon Lv 1 42 pts. 11,572
  7. Avatar for Tamison 7. Tamison Lv 1 35 pts. 11,572
  8. Avatar for Rav3n_pl 8. Rav3n_pl Lv 1 28 pts. 11,570
  9. Avatar for auntdeen 9. auntdeen Lv 1 23 pts. 11,570
  10. Avatar for MooMooMan 10. MooMooMan Lv 1 19 pts. 11,567

Comments

beta_helix Staff Lv 1

The solved native structure for CASP9 Puzzle 3, target T0527, is missing 3 residues in the middle of the protein.

You can see it here:
http://www.rcsb.org/pdb/explore/images.do?structureId=3MR0
in the image on the top left, the green region has a chain break (next to the cyan/blue area).

Because of this, we have used Rosetta to fill in the 3 missing residues so that the guide has a continuous chain (since there aren't that many different places where those 3 residues could go)
These 3 residues are Glycine 47, Histidine 48, & Arginine 49.

Also, as Madde pointed out in the forum:
http://fold.it/portal/node/988108#comment-8844

the C-terminal helix points straight out, even though it has a bunch of hydrophobic residues.