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724: Blind Electron Density Puzzle 7

Closed since almost 13 years ago

Advanced Overall Prediction Electron Density

Summary


Created
June 03, 2013
Expires
Max points
100
Description

This Electron Density puzzle is going to be as difficult as they get! Why? Because the automated methods for fitting electron density were unable to solve this structure using this density. But where computers fail, Foldit players triumph! Use all your Electron Density folding skills to fit the density as well as you can. As requested in the last Scientist Chat, we have added secondary structure predictions onto the starting model, with more details posted in the puzzle comments.

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Comments


beta_helix Staff Lv 1

As requested in the last Scientist Chat , here is the sequence logo predicted by the SAM server:

H = helix
E = sheet
C = loop (or coil)

The taller the letter at each position, the higher the probability of that specific secondary structure for that amino acid.

For example, the Valines at residues 7, 121 & 128 are predicted to be part of a sheet. However, the Valines at residues 36, 91, 102, 113 & 141-142 are highly predicted to be part of a helix!

The above predictions are already on the starting model (based on the horizontal lines in the above figure).
______________

Cutaway view hint:
If you hover your mouse over a segment and type shift-Q, the game will hide parts of the protein and cloud that are not in the same plane as that segment - it cuts down on how much cloud you see at one time and makes it easier to focus. Q returns to normal view.

alwen Lv 1

Not a lot of big hydrophobics in this one to use for landmarks, just that tryptophan at 80 to align the big helices.

It's possible the cloud is cut so we have the small helix from another copy.