I saved the given starting position in the ED puzzle and later loaded it as a guide. Rather than appearing in the same location relative to the density that it had at reset, the guide was translated and rotated relative to the density. I am able to align it to one segment of the protein, but what I really want is to have the guide aligned to the density in exactly position the protein had when saved. Otherwise it is useless as a guide. This should be true of any pose I save to use as a guide, not just the starting position. Also, if I load a guide from a save and later move the guide by aligning to protein, when I load that save as guide a second time it should move back to its saved position relative to the ED.
Thanks for letting us know about this, Susume.
Was this an issue on the previous ED puzzle, 807/810?
Does this happen in both devprev and main?
Hopefully we can figure out why this is happening!
I'm in devprev, win 7 64. I had not tried loading a guide on 807 or 810 - tried it just now.
807: loaded a save as guide, looked like it was lined up correctly to ED. Aligned it to a bad segment so it was no longer aligned to ED. Loaded a second save as guide, then reloaded first save as guide, then loaded a third save as guide - they were all way out of line - looks like all aligned to the one bad segment. Aligned guide to a good segment, then loaded each of the saves as guide again - now they are all aligned to the good segment - but no way to get them aligned to the ED itself, which is what's needed.
After loading about a dozen guides, client stops responding and has to be shut down - that happened in 813 too.
810: started with extended chain, loaded a save as guide - guide appeared very far from ED, overlapping residues 3-6 (though not aligned exactly to any one of them). Loaded a save (not as guide) - guide remained where it was. Loaded a save as guide; guide moved to be aligned to protein (probably around segs 3-6, though not sure). After this the behavior was the same as for 807.
The normal load as a guide alignes to the protein I think.
If I understand Susume correctly that is not what is wanted on ED puzzles, there we want the guide to be in the same ED position as the save was.
And as a consequence, guides should be locked in position in ED puzzles, so the alignment button would align the protein to the guide and not the guide to the protein.