Placeholder image of a protein
Icon representing a puzzle

1861: Symmetric Trimer Design: Buried Unsats

Closed since over 5 years ago

Intermediate Overall Design Symmetry

Summary

Created
July 08, 2020
Expires
Max points
100
Description

Design a symmetric protein trimer, with 3 identical chains that assemble together! This puzzle uses the Buried Unsats Objective, with a large penalty for buried polar atoms that can't make H-bonds. The H-bond Network Objective encourages players to build buried, satisfied H-bond networks at the interface between symmetric chains. H-bond networks are a great way to introduce polar residues at the interface, but it's important that all of the bondable atoms make hydrogen bonds! In this puzzle, there are no limits on the Complex Core, but we've included the Complex Core objective so players can see the core residues that can be incorporated into H-bond Networks.

Top groups

  1. Avatar for Foldit Staff 11. Foldit Staff 2 pts. 14,278
  2. Avatar for Hold My Beer 12. Hold My Beer 1 pt. 13,920
  3. Avatar for BOINC@Poland 13. BOINC@Poland 1 pt. 13,645
  4. Avatar for Trinity Biology 14. Trinity Biology 1 pt. 10,462
  5. Avatar for GBHS BMAH kids 15. GBHS BMAH kids 1 pt. 9,595
  6. Avatar for Italiani Al Lavoro 16. Italiani Al Lavoro 1 pt. 2,142
  7. Avatar for Rechenkraft.net 17. Rechenkraft.net 1 pt. 735
  8. Avatar for Window Group 18. Window Group 1 pt. 0
  1. Avatar for sgeldhof 41. sgeldhof Lv 1 19 pts. 16,297
  2. Avatar for cbwest 42. cbwest Lv 1 18 pts. 16,242
  3. Avatar for Deleted player 43. Deleted player pts. 16,168
  4. Avatar for pvc78 44. pvc78 Lv 1 16 pts. 16,104
  5. Avatar for inhtih 45. inhtih Lv 1 15 pts. 16,082
  6. Avatar for Hiro Protagonist 46. Hiro Protagonist Lv 1 15 pts. 16,080
  7. Avatar for Vinara 47. Vinara Lv 1 14 pts. 15,863
  8. Avatar for Jpilkington 48. Jpilkington Lv 1 13 pts. 15,832
  9. Avatar for ZeroLeak7 49. ZeroLeak7 Lv 1 13 pts. 15,791
  10. Avatar for fishercat 50. fishercat Lv 1 12 pts. 15,789

Comments

bkoep Staff Lv 1

Buried Unsats (max +500)
Penalizes polar atoms that cannot make hydrogen bonds, -150 points per atom (not including symmetric copies).

Core Existence: Monomer (max +1700 +1600)
Ensures that at least 17 16 residues are buried in the core of the monomer unit.

Core: Complex (max +0)
Awards no bonuses or penalties. Click Show to see which residues count as "Core" for the H-bond Network objective.

H-bond Network (max +1800)
Rewards networks that comprise at least 3 H-bonds involving core residues.
Between 1 and 9 H-bonds should cross the interface between symmetric units.
Networks must be at least 75% satisfied (i.e. 75% of all bondable atoms in a network must make a H-bond).

SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, ALA, SER, THR in helices.

Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.