1903: Two-sided Interface Design

Closed since over 5 years ago

Summary

• Created: October 14, 2020
• Expires: October 21, 2020 at 23:00 UTC
• Max points: 100

Description

Note: This puzzle was closed early due to problems in the puzzle setup. A corrected version has been posted as Puzzle 1903b.



Design an interface between two protein chains! This puzzle is a little different from our regular design puzzles. The starting structure includes two helical hairpins that are frozen in place and separated from one another. We want to build on these hairpins to create a binding interface between the two protein chains. However, the starting position of the hairpins is important, so players cannot move the hairpins closer to one another. The only way to make the two chains bind will be to fold up a brand new interface between them.



As you build on the two hairpins, it will be important to make a strong core with each chain, so that the chains fold up correctly. It will also be important to have a strong core at the interface where the two chains come together, so that they bind each other tightly. Keep an eye on the Core Existence Objective, which tracks all of these core residues together. Players may add up to 20 additional residues at a cost of 55 points per residue. See the puzzle comments for Objective details.

Top groups

• 1. Gargleblasters 100 pts. 18,285
• 2. Go Science 33 pts. 15,828
• 3. Anthropic Dreams 8 pts. 13,944
• 4. Contenders 2 pts. 0
• 5. Beta Folders 1 pt. 0
• 6. Marvin's bunch 1 pt. 0

Top soloists

• 1. Enzyme 100 pts. 18,285
• 2. donuts554 73 pts. 15,828
• 3. Galaxie 52 pts. 13,944
• 4. Beany 36 pts. 13,606
• 5. TastyMunchies 24 pts. 10,120
• 6. dcrwheeler 16 pts. 8,044
• 7. Ashrai 10 pts. 7,633
• 8. ZeroLeak7 6 pts. 0
• 9. Formula350 1 pt. 0
• 10. malphis 2 pts. 0

Comments

[bkoep]

Residue Count (max +1100)
Penalizes added residues at a cost of 55 pts per residue. Players may add up to 20 residues, for a total of 200.

Buried Unsats (max +500)
Penalizes polar atoms that cannot make hydrogen bonds, -150 points per atom (not including symmetric copies).

Core Existence: Total (max +5000)
Ensures that at least 25% of residues are buried in the core of the entire solution.

Interaction Energy (max +500)
Monitors that all large PHE, TYR, and TRP residues are scoring well.

SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, SER, THR in helices.

Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.

[Formula350]

ATTENTION PLAYERS

1903 has a slight bug currently, which causes Blueprints to move the Locked Helices on Segments 1-90 bundle. I've let bkoep know, BUT in the meantime…

HERE IS WHAT YOU NEED TO DO IN ORDER TO PLAY
(1) Reset your puzzle
(2) Open View Options
(3) Enable: 'Show backbone pins'
(4) Rotate camera to so you can see where the end of the first-chains Loops end (Segment 90),
(5) Click on the Pin at the end

This will cause the pin to jump to where it is supposed to be.
NOW you can go about playing the puzzle and using Blueprints.
:)