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1977: Symmetric Tetramer Design: H-bond Networks

Closed since almost 5 years ago

Summary

Created: April 07, 2021
Expires: April 14, 2021 at 23:00 UTC
Max points: 100

Description

Design a symmetric protein tetramer, with 4 identical chains that assemble together! This puzzle includes a Secondary Structure Objective, so no more than 50% of your design can form helices. The H-bond Network Objective encourages players to build buried, satisfied H-bond networks at the interface between symmetric chains. H-bond networks are a great way to introduce polar residues at the interface, but it's important that all of the bondable atoms make hydrogen bonds! We've also adjusted the H-bond Network Objective so that poor-scoring H-bonds may not contribute to networks; poor-scoring H-bonds will be displayed in red. This puzzle uses the Buried Unsats Objective, with a large penalty for buried polar atoms that can't make H-bonds. In this puzzle, there are no limits on the Complex Core, but we've included the Complex Core objective so players can see the core residues that can be incorporated into H-bond Networks.

Top groups

11. BOINC@Poland 2 pts. 15,571
12. Team China 1 pt. 15,369
13. Richard Dawkins Foundation 1 pt. 12,195
14. Czech National Team 1 pt. 12,030
15. Coastal Biochemistry 1 pt. 1,249
16. Mojo Risin' 1 pt. 951
17. Trinity Biology 1 pt. 0
18. Foldit Staff 1 pt. 0
19. Window Group 1 pt. 0

11. silent gene Lv 1 62 pts. 20,794
12. Skippysk8s Lv 1 59 pts. 20,731
13. akaaka Lv 1 56 pts. 20,730
14. Galaxie Lv 1 53 pts. 20,382
15. fiendish_ghoul Lv 1 50 pts. 20,369
16. hpaege Lv 1 47 pts. 20,306
17. frood66 Lv 1 45 pts. 20,053
18. johnmitch Lv 1 43 pts. 19,926
19. Mike Cassidy Lv 1 40 pts. 19,869
20. georg137 Lv 1 38 pts. 19,860

Comments

bkoep Staff Lv 1

April 07, 2021

Buried Unsats (max +500)
Penalizes polar atoms that cannot make hydrogen bonds, -200 points per atom (not including symmetric copies).

Core Existence: Monomer (max +2200)
Ensures that at least 22 residues are buried in the core of the monomer unit.

Core: Complex (max +0)
Awards no bonuses or penalties. Click Show to see which residues count as "Core" for the H-bond Network objective.

H-bond Network (max +2400)
Rewards networks that comprise at least 2 H-bonds involving core residues.
Between 1 and 12 H-bonds should cross the interface between symmetric units.
Networks must be at least 75% satisfied (i.e. 75% of all bondable atoms in a network must make a H-bond).

Interaction Energy (max +500)
Monitors that all large PHE, TYR, and TRP residues are scoring well.

SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, ALA in helices.

Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.

Secondary Structure (max +500)
No more than 50% of residues may form helices. Extra helices are penalized at 10 points per residue.