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2672; Electron Density Reconstruction 139

Closed since 5 months ago

Summary

Created
September 30, 2025
Expires
Max points
0
Description

The structure of this protein has already been solved and published, but close inspection suggests that there are some problems with the published solution. We'd like to see if Foldit players can use the same electron density data to reconstruct a better model.

Sequence
MRLLSSLSGSSVSSDAEEYQPPIWKSYLYQLQQEAPRPKRIICPREVENRPKYYGREFHGIISREQADELLGGVEGAYILRESQRQPGCYTLALRFGNQTLNYRLFHDGKHFVGEKRFESIHDLVTDGLITLYIETKAAEYISKMTTNPIYEHIGYATLLREKVSRRLSRSKNEPRKTNVTHEEHTAVEKISSLVRRAALTHNDNHFNYEKTHNFKVHTFRGPHWCEYCANFMWGLIAQGVRCSDCGLNVHKQCSKHVPNDCQPDLKRIKKVYCCDLTTLVKAHNTQRPMVVDICIREIEARGLKSEGLYRVSGFTEHIEDVKMAFDRDGEKADISANVYPDINIITGALKLYFRDLPIPVITYDTYSKFIDAAKISNADERLEAVHEVLMLLPPAHYETLRYLMIHLKKVTMNEKDNFMNAENLGIVFGPTLMRPPEDSTLTTLHDMRYQKLIVQILIENEDVLF

Top groups

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    1. hada Lv 1
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Comments

LociOiling Lv 1

This puzzle is a match for PDB 1XA6. The experiment for 1XA6 ended up with a long section of missing residues in the middle of the chain. As a result, Foldit recipes see two chains, one before the gap and one after the gap.

LociOiling Lv 1

Major oops, thanks to Anfinsen_slept_here for pointing out the puzzle has a pucker at 141 and 142. So in the puzzle, segment is bonded to segment 142, which is not correct.

AA Edit and similar recipes see two chains due to the distance between the two segments/residues.

The incorrect bonding means everything will be pulled into an unrealistic position, trying to correct the very bad ideality and other problems created by the pucker.

Bruno Kestemont Lv 1

Ideed ARG 141 to TYR142 is similar to PDB ARG141 to PDB TYR209. Trying to reconstruct the link is artificial AND destroys a part of the ED match.

I wonder if our results will be usefull for science ?