Foldit Puzzles

2156: TGF Binder Design: Round 18

Design a protein that can bind to the TGF-beta receptor! This puzzle has a reduced BUNS penalty so that players can focus on other Objectives--but don't forget about BUNS completely! AlphaFold predictions are available for your designed binder. Once you've designed a binder for the target, upload your solution for AlphaFold using the AlphaFold prediction tool. AlphaFold will predict the structure of your binder chain only (i.e. in the unbound state, in the absence of the target). If you load this prediction, then Foldit will attempt to align the prediction with your solution (i.e. in the bound state, making an interface with the target). If you continue working off of the AlphaFold prediction, you may need to make adjustments at the interface where the binder interacts with the target.

2155: 588-residue Electron Density Reconstruction 8

Note: Due to a bug in cutpoint behavior, this puzzle was closed early land reposted as Puzzle 2155b. Players may save solutions from this puzzle and load them into the reposted puzzle.

2155b: 588-residue Electron Density Reconstruction 8

Note: This puzzle replaces the original Puzzle 2155, which was closed early due to a bug in cutpoint behavior. Players may load solutions from the original puzzle.

2154: KLHDC2 ligand design: Round 7

Design a small molecule which can bind to KLHDC2! We don't want you to modify the protein, but instead use the small molecule design tools to build new small molecules which fit into the KLHDC2 binding pocket.

2153: Symmetric Tetramer Design with AlphaFold Predictions

Design a symmetric protein tetramer, with 4 identical chains of 90 residues each! This puzzle enables AlphaFold predictions for the monomer subunit of your design, so you can upload your solution for AlphaFold using the AlphaFold prediction tool. AlphaFold will predict the structure of your monomer subunit only (i.e. in the unbound state, in the absence of other symmetric copies). If you load this prediction, then Foldit will attempt to align the prediction with your solution. If you continue working off of the AlphaFold prediction, you may need to make adjustments at the interface where the monomer subunit interacts with symmetric copies.

2151: KLHDC2 ligand design: Round 6

Design a small molecule which can bind to KLHDC2! We don't want you to modify the protein, but instead use the small molecule design tools to build new small molecules which fit into the KLHDC2 binding pocket.

2152: CASP11 Refinement Puzzle (TR760) with Trim Tool

Way back in 2014 we posted this same refinement puzzle and found that it was a bit too large to run in Foldit at the time, with a size of 201 residues.

Design of the Month: May 2022

This sandbox puzzle features a CD47 binder design by alcor29 in Puzzle 2132. The binding interface demonstrates a fine balance of hydrophobics (for tight binding) and polar sidechains (for folding and BUNS satisfaction).

2150: CD47 Binder Design: Round 9

Design a protein that can bind to CD47! This puzzle has a reduced BUNS penalty so that players can focus on other Objectives--but don't forget about BUNS completely! AlphaFold predictions are available for your designed binder. Once you've designed a binder for the target, upload your solution for AlphaFold using the AlphaFold prediction tool. AlphaFold will predict the structure of your binder chain only (i.e. in the unbound state, in the absence of the target). If you load this prediction, then Foldit will attempt to align the prediction with your solution (i.e. in the bound state, making an interface with the target). If you continue working off of the AlphaFold prediction, you may need to make adjustments at the interface where the binder interacts with the target.

2149: Revisiting Puzzle 139: Rosetta Decoy 3

This is a throwback puzzle to the early days of Foldit. Ubiquitin is a well-known protein that helps to regulate the natural turnover of proteins in the cell, and this starting structure is a model of the protein produced by Rosetta. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been and to provide newer players with puzzles that are still scientifically relevant.