Foldit Puzzles

2112: Symmetric Tetramer Design with AlphaFold Predictions

Design a symmetric protein tetramer, with 4 identical chains of 80 residues each! This puzzle enables AlphaFold predictions for the monomer subunit of your design, so you can upload your solution for AlphaFold using the AlphaFold prediction tool. AlphaFold will predict the structure of your monomer subunit only (i.e. in the unbound state, in the absence of other symmetric copies). If you load this prediction, then Foldit will attempt to align the prediction with your solution. If you continue working off of the AlphaFold prediction, you may need to make adjustments at the interface where the monomer subunit interacts with symmetric copies.

Design of the Month: February 2022

This sandbox puzzle features a symmetric protein design by fiendish_ghoul from Puzzle 2096: Symmetric Trimer Design. The monomer subunit mixes alpha helices and beta sheets, and presents a well-satisfied H-bond network at the symmetric interface. For more, check out Foldit Lab Report #29 on YouTube! This sandbox puzzle is non-competitive and will not award any points.

2111: CD22 Binder Design: Round 1

Note: This puzzle was closed early due to an error that allowed parts of the target chain to move. This puzzle has been reposted as Puzzle 2111b.

2111b: CD22 Binder Design: Round 1

Note: This puzzle replaces Puzzle 2111 which mistakenly allowed parts of the target chain to move. Players may not load work from the original Puzzle 2111.

2110: Revisiting Puzzle 114: Black Mamba

This is a throwback puzzle to the early days of Foldit. This toxin is produced in the intestines of the African black mamba. The protein contains ten cysteines that oxidize to form five disulfide bonds. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been and to provide newer players with puzzles that are still scientifically relevant.

2109: Symmetric Tetramer Design with AlphaFold Predictions

Design a symmetric protein tetramer, with 4 identical chains of 60 residues each! This puzzle enables AlphaFold predictions for the monomer subunit of your design, so you can upload your solution for AlphaFold using the AlphaFold prediction tool. AlphaFold will predict the structure of your monomer subunit only (i.e. in the unbound state, in the absence of other symmetric copies). If you load this prediction, then Foldit will attempt to align the prediction with your solution. If you continue working off of the AlphaFold prediction, you may need to make adjustments at the interface where the monomer subunit interacts with symmetric copies.

2108: Electron Density Reconstruction 4

The structure of this protein has already been solved and published, but close inspection suggests that there are some problems with the published solution. We'd like to see if Foldit players can use the same electron density data to reconstruct a better model. In addition to the protein chain, puzzle includes three sulfate ions that are fixed in place.

2107: Revisiting Puzzle 113: White Birch

This is a throwback puzzle to the early days of Foldit. This protein is an allergen produced by the white birch tree. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been and to provide newer players with puzzles that are still scientifically relevant.

2106: Design with Neural Net Objective

Design a protein with the help of the new Neural Net Objective! The Neural Net Objective can help identify parts of your protein that should be mutated to improve AlphaFold prediction confidence. After you use the AlphaFold tool to get a prediction, click "Show Neural Net Objective" to color your protein based on local distogram analysis. To improve AlphaFold prediction confidence, try mutating residues that are colored red by the Neural Net Objective. Players should aim for an AlphaFold confidence and similarity of 80% or more.

2105: Symmetric Trimer Design with AlphaFold Predictions

Design a symmetric protein trimer, with 3 identical chains of 90 residues each! This puzzle enables AlphaFold predictions for the monomer subunit of your design, so you can upload your solution for AlphaFold using the AlphaFold prediction tool. AlphaFold will predict the structure of your monomer subunit only (i.e. in the unbound state, in the absence of other symmetric copies). If you load this prediction, then Foldit will attempt to align the prediction with your solution. If you continue working off of the AlphaFold prediction, you may need to make adjustments at the interface where the monomer subunit interacts with symmetric copies.