Foldit Puzzles

1104: Revisiting Puzzle 79: Pheromone

This is a throwback puzzle to the early days of Foldit. This pheromone protein is secreted by the protist E. raikovi to initiate conjugation, the exchange of genetic material between two cells. This protein contains ten cysteine residues that oxidize to form five disulfide bonds. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been.

1103: Asymmetric Trimer Design: Hydrogen Bond Network

This is a repost of the trimer from Puzzle 1087, but now the three chains will NOT behave symmetrically! This means that each chain may have a different sequence, and players must design three separate interfaces. Satisfy the Hydrogen Bond Network filter by mutating residues to form HBonds between the Red and Blue polar atoms on the sidechains.

1102: Integrated Abeta Design

This large protein (291 residues!) is based on a prenyltransferase protein from Streptomyces; however, we've inserted the sequence of Aβ into residues 267-291. We're asking Foldit players to redesign this protein so that it folds up normally with the integrated Aβ! Such a protein could serve as a starting point for the design of an Aβ binder.

1101: Revisiting Puzzle 77: Copper Chaperone

This is a throwback puzzle to the early days of Foldit. This protein binds copper ions so that they may be transported safely to the cell compartments and enzymes that require them. This protein has three cysteine residues, although they remain in the reduced state within the cell, and will not form disulfide bonds. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been.

1100: Vancomycin Binder Design: Hydrogen Bond Network

This is another filter featuring the Hydrogen Bond Network filter. Instead of two protein chains, the puzzle includes a protein and a ligand, similar to Puzzle 1076. Redesign the binding pocket of the ligand to include a hydrogen bond network! This puzzle has four different starting structures, all with slightly different residues at the binding site; resetting the puzzle will cycle through the different models. See puzzle comments for filter details.

1099: Revisiting Puzzle 76: Plant Seed Protein

This is a throwback puzzle to the early days of Foldit. This storage protein is used to stockpile amino acids for future use, and is found at high concentration in the seeds of certain plants. This particular protein was used by scientists in 2011 to collect the highest-resolution crystal structure to date. This protein contains six cysteine residues that oxidize to form three disulfide bonds. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been.

1098: De-novo Freestyle 51: Round 2

This is Round 2 for Puzzle 1094. You will be able to load in your manual saves from 1094 and use them as a starting point here. This puzzle has been opened up to allow for sharing and the use of all scripts. NOTE: If you did not manually save a solution in puzzle 1094, you can go back to 1094, manually save it, and the solution should appear in your manual saves for this puzzle.

1097: Asymmetric Hydrogen Bond Networks

This is our second puzzle featuring the new Hydrogen Bond Network filter. This puzzle contains two chains, however they are not symmetric—players will need to design both sides of the interface between the two chains! We've provided you with a fixed backbone that can make a number of HBond networks. Mutate the sequence to form HBonds between the Red and Blue polar atoms on the sidechains. Be sure to check out the recent blog post for more information!

1096: 70 Residue Monomer Design: Best Fragments

This puzzle uses a more stringent fragment filter than previous design puzzles. See the puzzle comments for filter details. The Baker Lab will run folding predictions on your solutions for this puzzle, and those that perform well will be synthesized in the lab. Remember, you can use the Upload for Scientists button for up to 5 designs that you want us to look at, even if they are not the best-scoring solutions!

1095: Revisiting Puzzle 75: Antifreeze Protein

This is a throwback puzzle to the early days of Foldit. This eelpout protein binds nucleated ice crystals to inhibit their growth. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been.