Foldit Puzzles

1058: Player-Solution Trimer Redesign A

See if you can improve this Foldit player-designed protein! The starting structure is a Scientist-Shared solution designed by Bletchley Park, mimi, and spvincent in Puzzle 1052. We don't want players to stray too far from the initial design, so there's an Exploration filter to keep solutions close to the starting structure. The Baker Lab will run folding predictions on your solutions for this puzzle, and those that perform well will be synthesized in the lab. Remember, you can use the Upload for Scientists button for up to 5 designs that you want us to look at, even if they are not the best-scoring solutions!

1057: 26 Residue Peptide Design: More Sheets!

Like Puzzle 1048, this puzzle is aimed at designing short peptides that fold into stable structures, now with an emphasis on designed sheets! See the puzzle comments for more information about filters. The Baker lab will run folding predictions on your best designs, and the the ones that perform well will be synthesized and further validated for structural stability. Remember, you can use the Upload for Scientists button for up to 5 designs that you want us to look at, even if they are not the best-scoring solutions!

1056: Revisiting Puzzle 61: Designer Protein Top7

This is a throwback puzzle to the early days of Foldit. This protein was designed by the Baker Lab in 2003, and has a topology unlike any natural protein yet discovered. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been.

1055: Blind Electron Density 10: Autobuild Model

This is the same target that players folded in 1051: Symmetric De-novo Freestyle 48, now with electron density! The two chains are not constrained by symmetry in this puzzle, although we still expect them to adopt mostly-symmetric folds. The starting model is a solution built with an automated method—see if you can improve it! Players will not be able to load in solutions from Puzzle 1051.

1054: Vancomycin Binder: Shape Complementarity

Design a binding pocket for vancomycin! Antibiotic resistance is an increasing problem and one of the last resorts for treating inflammation is the cyclic peptide vancomycin. Hence there is a general interest to generate proteins that can bind vancomycin and upon binding generate a signal. With this technology it would be possible to test patients that have been treated with antibiotics if they still have small amounts left before they leave e.g. a hospital. The aim of this puzzle is to optimize the interaction between a protein and the small molecule vancomycin.

1053: Revisiting Puzzle 60: Beta Barrel

This is a throwback puzzle to the early days of Foldit. This protein binds fatty acids in intestinal cells. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been.

1052: 80 Residue Symmetric Trimer Design

This puzzle again uses separate "Core Existence" filters for the monomer unit and the symmetric complex. Click the "Show" checkbox beneath the Core Existence filter to see which residues have been identified as Core (orange), Boundary (green), or Surface (blue). There are several other filters in effect; see the puzzle comments for details. The Baker Lab will run folding predictions on your solutions for this puzzle, and those that perform well will be synthesized in the lab. Remember, you can use the Upload for Scientists button for up to 5 designs that you want us to look at, even if they are not the best-scoring solutions!

1051: Symmetric De-novo Freestyle 48

This is a symmetric dimer that was recently designed by Baker Lab scientists. See if you can find the correct fold! The secondary structure predictions are provided on the starting model, with more details posted in the puzzle comments.

1050: Revisiting Puzzle 59: TCR Binding Protein

This is a throwback puzzle to the early days of Foldit. This small, intracellular domain binds to the CD2 T cell receptor (TCR), and plays a critical role in T cell activation during the immune response. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been.

1049: VipB Cryo-EM Density

This is a repost of Puzzle 1045, now with normal density scoring. Load in your solutions from Puzzle 1045 and see if you can improve them to fit the density! This is a small domain of the VipB protein, which is a component of the type VI secretion system in V. cholerae. NOTE: If you did not manually save a solution in Puzzle 1045, you can go back to 1045, manually save it, and the solution should appear in your manual saves for this puzzle.