Foldit Puzzles

641: CASP ROLL Target R0020

CASP ROLL is back! We are providing you with 3 templates for now (in hope that one of them is correct), and will give you with some server predictions once they are released. More details about this CASP target are in the puzzle comments.

640: Frizzled Design Puzzle

You've seen this design puzzle before, but look more closely! The Frizzled protein occurs in a number of forms. Last time we gave you a form of Frizzled with a narrow pocket. This time we are giving you a form of Frizzled with a wider pocket. There are a couple of rules: You can mutate any residue on the helix but none on Frizzled and the small binding helix must stay in helical shape. We've also added a new filter that we call "Residue Interaction Energy". Natural proteins avoid putting Tryptophan, Phenylalanine, and Tyrosine on the surface. However, Foldit often gives a score bonus if you load up the surface of your design with Tryptophan. The Residue Interaction Energy filter helps to counteract that, helping you make better designs. Enjoy and thanks for all of your efforts!

639: De-novo Freestyle 17

This currently unsolved short protein does not have any templates that we can use, so to prepare for CASP ROLL we are giving it to you as an extended chain. Use all your skills to fold it up as best as you can. The secondary structure predictions are provided on the starting model, with more details posted in the puzzle comments.

638: 40 Residue Symmetric Tetramer Freestyle

We're moving up to a 40 residue version of our previous ring-tetramer puzzle to go along with the dimer of timders. Increasing the number of residues makes your designs more likely to fold up in nature. Remember, a symmetric protein relies not only on how well-folded each chain is, but also on how well they interface together.

637: De-novo Freestyle 16

This currently unsolved short protein does not have any templates that we can use, so we are giving it to you as an extended chain. Use all your skills to fold it up as best as you can. The secondary structure predictions are posted in the puzzle comments.

636: 40 Residue Symmetric Dimer of Dimers Freestyle

We're moving up from the 30 residue dimer of dimers puzzle that we posted before, since increasing the length makes the produced designs more likely to fold up in nature. Remember, a symmetric protein relies not only on how well-folded each chain is, but also on how well they interface together. This dimer of dimers has two fundamentally different interfaces: the interface between chains A and B, and the interface between dimer AB and its partner, CD. We're really excited with some of the designs we've seen so far, and we're eager for more!

635: De-novo Freestyle 15

This currently unsolved protein does not have any templates that we can use, so we are giving it to you as an extended chain. Use all your skills to fold it up as best as you can. The secondary structure predictions are posted in the puzzle comments, and included in the starting structure as well.

634: Electron Density Puzzle 11

In this electron density puzzle, we're giving you some more realistic, noisy density. You'll have to use the electron density as a guide in order to find the correct fold. Try to find the best fit for the protein inside of the guide - but remember that since the density is noisy, it isn't always correct. We have posted the secondary structure predictions in the puzzle comments. Get started by completing the electron density intro level (5-3).

633: Return of the Tissue Regeneration Design Puzzle

We are giving you a 10 residue helix positioned over a hydrophobic groove where binding occurs in nature. This native binding region in marked by unfrozen side chains. Try to look for favorable hydrophobic interactions in this groove while maintaining the helix. If you are new to Foldit, make sure you have completed Intro Puzzle 5-2, and 7-1 through 7-4 before trying this puzzle. More details in the puzzle comments. Note: you will need the latest update to play this puzzle.

Beginner Puzzle: Streptococcal Protein

The fold of this Streptococcal Protein is trapped in a local minimum. Although it looks good, it is incorrect. This is a short 57-residue protein for new players to try out after completing the Introductory Puzzles. The native is shown as a guide on this puzzle, so try using everything you have learned in the Intro levels to match the native fold.