Foldit Puzzles

773: De-novo Freestyle 29: Hand-folding Round

We are giving you another currently unsolved protein as an extended chain. This round only GUI scripts are allowed and sharing has been disabled. After this puzzle expires, the puzzle will be re-posted and LUA scripts and sharing will be allowed. You'll be able to load in your solutions from this puzzle and use scripting and sharing. The secondary structure predictions are provided on the starting model, with more details posted in the puzzle comments.

772: Unsolved Salmonella Bacteriophage: Cryo-electron Density with Alignments: Round 2

The salmonella bacteriophage protein is back for another round! After reviewing your solutions from Puzzle 765, we've decided to repost the high-quality electron density cloud with alignments. In addition to the starting structures by Galaxie, meatexplosion, and karstenw, this puzzle also includes a high-scoring solution by Batz. You can use the alignment tool to compare these solutions as well as a structure that was determined by another lab (PDB entry 3j40), although we're fairly certain this solution is inaccurate. You can load in your solutions from the previous puzzles (737, 740, 749, 756, and 765) and can find more details in the puzzle comments.

771: De-novo Freestyle 28: Round 2

This is Round 2 for Puzzle 767. You will be able to load in your manual saves from 767 and use them as a starting point here. This puzzle has been opened up to allow for sharing and the use of all scripts. NOTE: If you did not manually save a solution in puzzle 767, you can go back to 767, manually save it, and the solution should appear in your manual saves for this puzzle.

770: Revisiting Puzzle 73: Polycystein

This is a throwback puzzle to the early days of Foldit. This protein has 80 sidechains, and it's a membrane protein. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been. Players will NOT be able to load in any previous solutions for these puzzles.

768: Revisiting Puzzle 72: Glycolysis

This is a throwback puzzle to the early days of Foldit. This short sequence is a domain of a succinyltransferase enzyme in E. coli. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been. Players will NOT be able to load in any previous solutions for these puzzles.

769: Quick Loop Redesign

This is a puzzle where you can redesign part of a player-designed protein. This is the subunit of a symmetric tetramer designed by jamiexq, karstenw, and markm457 for Puzzle 725. Baker lab simulations suggest that one of the beta strands is unlikely to form because the loops on either end prefer different conformations. Help us redesign these loops to find a solution that will fold up properly! You have one additional residue that you can insert in the protein, but most of the protein is frozen in this puzzle. This puzzle will only be active for three days!

767: De-novo Freestyle 28: Hand-folding Round

We are giving you another currently unsolved protein as an extended chain. This round only GUI scripts are allowed and sharing has been disabled. After this puzzle expires, the puzzle will be re-posted and LUA scripts and sharing will be allowed. You'll be able to load in your solutions from this puzzle and use scripting and sharing. The secondary structure predictions are provided on the starting model, with more details posted in the puzzle comments.

766: Revisiting Puzzle 71: Crystallin

This is a throwback puzzle to the early days of Foldit. This is an eye lens protein. We are revisiting old Foldit puzzles so we can see how useful the recent additions to the game have been. Players will NOT be able to load in any previous solutions for these puzzles.

765: Unsolved Salmonella Bacteriophage: Cryo-electron Density with Alignments

This unsolved bacteriophage protein is the same protein that you've been working on in puzzles 737, 740, 749, and 756. We've analyzed the top solutions from the latest puzzle, in which you tried to fold an extended chain into an electron density cloud, and we want to see if you can keep improving on this structure. This puzzle includes the three most promising Foldit solutions (by Galaxie, meatexplosion, and karstenw) as starting structures, and the electron density cloud is a little more coarse. We're also providing you with a structure that was determined by another lab (PDB entry 3j40), although we're fairly certain this solution is inaccurate. You can load in your solutions from the previous puzzles (737, 740, 749, and 756) and can find more details in the puzzle comments.

764: 45 Residue Pentamer Design

This is a 45 residue symmetric pentamer design puzzle; there will be five symmetric chains to manage. Note that the score of a symmetric protein relies not only on how well-folded each chain is, but also on how well the chains interface together. See the blog post about this for more information. There are 2 filters enabled on this puzzle: the Residue IE Score monitors that all PHY, TYR, and TRP residues are scoring well, and the Core Exists filter checks that at least 30% of the residues are buried in the core of your design. The Baker Lab will run folding simulations on your solutions for this puzzle, and those that perform well will be synthesized in the lab. Remember, you can use the Upload for Scientists button for up to 3 designs that you want us to look at, even if they are not the best-scoring solutions.