Foldit Puzzles

724: Blind Electron Density Puzzle 7

This Electron Density puzzle is going to be as difficult as they get! Why? Because the automated methods for fitting electron density were unable to solve this structure using this density. But where computers fail, Foldit players triumph! Use all your Electron Density folding skills to fit the density as well as you can. As requested in the last Scientist Chat, we have added secondary structure predictions onto the starting model, with more details posted in the puzzle comments.

725: Quick 60 Residue Tetramer Redesign

This is a 60 residue symmetric tetramer design puzzle. The starting structure is a solution to Puzzle 691 that was shared with us by MurloW. The scientists at the Baker Lab like the topology of this solution, but simulations predict that this amino acid sequence will not fold up properly from an extended chain. Help us redesign this solution into a folding protein! The ResidueIE and "Core Exists" filters will make sure PHY, TYR, and TRP residues are scoring well, and that the protein core includes at least 35% of the residues. We look forward to seeing your mutations to this fold, although you are free to redesign the entire protein in this puzzle.

723: Server models for R0034

We are giving you non-Rosetta server predictions for this CASP ROLL target. Resetting the puzzle will cycle through 5 different Zhang Server predictions and they are available in the Alignment Tool so you can use partial threading between them. Please try out each start, as the topology that initially scores poorly may actually be the closest to the native and will eventually score better than everything else. The secondary structure predictions are provided on the extended chain in the Alignment Tool... for real, this time!

722: Design the Core Round 2

This is Round 2 for Puzzle 718. You will be able to load in your manual saves from 718 and use them as a starting point here. This puzzle has been opened up to allow for sharing and the use of all scripts. See if you can improve your model from the first round using these tools! NOTE: If you did not manually save a solution in puzzle 718, you can go back to 718, manually save it, and the solution should appear in your manual saves for this puzzle.

721: Solo Hand-Folding Sepsis Puzzle 3

We are giving you Museka's solution from Puzzle 684 as a starting model to work on, we have deleted 1 residue (you may delete even more). The sugar molecule bound to this protein is part of a larger carbohydrate structure that is a component of the bacterial cell surface; it is not an isolated sugar as implied by the crystal structure. Therefore, it is crucial that the top portion of the sugar be free of obstructions from the protein; so your designs should only access the sugar from the bottom and sides. More details in the puzzle comments. Because this is a Hand-Folding round, only GUI scripts are allowed and sharing has been disabled.

719: Two chains of Insulin Mutant Repost

We are reposting puzzle 716, but this time with the new Disulfide Count Score Filter that gives you a bonus for forming bridges. More info about Disulfide Bridges in Foldit here: http://foldit.wikia.com/wiki/Disulfide_Bridge. Once formed, you'll need to click "Show Bonds (sidechain)" to view them.

720: Quick Puzzle: 76 Residue Dimer

This is 76 residue symmetric dimer design puzzle, much like Puzzle 708. There are two flters enabled on this puzzle. The ResidueIE filter ensures PHY, TYR, and TRP residues are scoring well. The "Core Exists" filter ensures the number of residues in the core is at least 30%. We're starting you with johnmitch's monomer solution to Puzzle 687, but we've mutated all residues to isoleucine. You'll have to make your own mutations and design a well-packed dimer interface to increase your score. This is just a starting point, of course; you are free to redesign the entire protein in this puzzle.

718: Hand-Folding Flu Puzzle: Design the Core

In this puzzle we are trying to create a new better core for one of the Baker Lab flu proteins. We know what the key interface residues should be, that's why you can't change them, but a new core may increase the stability of the protein. Ultimately a more stable protein might translate into a better protein therapeutic. We don't want you to drift too far from the initial topology, so this puzzle has an RMSD condition. You might want to mutate the core of the protein before you wiggle to not violate this condition. Because this is a Hand-Folding round, only GUI scripts are allowed and sharing has been disabled. More details about all this in the puzzle comments.

717: CASP ROLL Target R0034

CASP ROLL started up again, but this is the first target that isn't too large for Foldit. We are giving you the 5 Rosetta Server models to work on. Resetting the puzzle will cycle through them, please try out all 5 starting points. The secondary structure predictions are provided on the extended chain in the Alignment Tool, with more details posted in the puzzle comments.

714: 5-year-anniversary puzzle: 2012

Puzzle "Blind Electron Density 2" is from our 5th year of Foldit. This was our second electron density puzzle with real data. Try to fit the protein into the density to get the best score!